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matchmaker gold yeast  (TaKaRa)


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    TaKaRa matchmaker gold yeast
    Matchmaker Gold Yeast, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+hybrid+system/Matchmaker+Gold+Yeast+Two-Hybrid+System/pm42127116-319-8-14
    Average 99 stars, based on 4235 article reviews
    matchmaker gold yeast - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Construct:

    Article Title: The BnTFL1-BnJAM3-BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus.
    Article Snippet: A prey cDNA library was constructed in the pGADT7 vector by OE Biotech Co., Ltd (Shanghai, China) using ZS11 developing seeds at 24–36 DAP. .. Both the bait construct and the prey library were cotransformed into Y2HGold Chemically-Library Competent Cells (Cat# YCL1002, Weidi, China) using the Matchmaker Gold Yeast Two-Hybrid System (Cat# 630489, TaKaRa, Japan). .. Positive protein–protein interactions were selected on synthetic defined (SD) medium lacking leucine, tryptophan, histidine, and adenine (SD − Leu/ − Trp/ − His/ − Ade; Cat# PM211, Coolaber, China).

    Y2H Assay:

    Article Title: Antiviral RNAi defective 4 (AVI4) encodes a lysophospholipid acyltransferase coopting AVI2 and ATG8a to promote antiviral immunity in Arabidopsis.
    Article Snippet: Autophagosomes labeled 471 GFP-ATG8a in plant cells located in maturation zone of the primary root were observed 472 using a Leica TCS SP8 confocal microscope, GFP was imaged with 488-nm excitation 473 and a detection window of 500 to 530 nm. .. 474 Yeast two-hybrid assay 475 The coding sequences of genes were cloned into the pGBKT7 and pGADT7 vectors, 476 respectively, to investigate protein-protein interactions using the Matchmaker Gold 477 Yeast Two-Hybrid System (Clontech, CA, USA). ..

    Article Title: The GATA8-GRF5-XTH9 feed-forward loop regulates cell size in poplar
    Article Snippet: .. The yeast two-hybrid assay was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA). ..

    Clone Assay:

    Article Title: Antiviral RNAi defective 4 (AVI4) encodes a lysophospholipid acyltransferase coopting AVI2 and ATG8a to promote antiviral immunity in Arabidopsis.
    Article Snippet: Autophagosomes labeled 471 GFP-ATG8a in plant cells located in maturation zone of the primary root were observed 472 using a Leica TCS SP8 confocal microscope, GFP was imaged with 488-nm excitation 473 and a detection window of 500 to 530 nm. .. 474 Yeast two-hybrid assay 475 The coding sequences of genes were cloned into the pGBKT7 and pGADT7 vectors, 476 respectively, to investigate protein-protein interactions using the Matchmaker Gold 477 Yeast Two-Hybrid System (Clontech, CA, USA). ..

    Protein-Protein interactions:

    Article Title: Antiviral RNAi defective 4 (AVI4) encodes a lysophospholipid acyltransferase coopting AVI2 and ATG8a to promote antiviral immunity in Arabidopsis.
    Article Snippet: Autophagosomes labeled 471 GFP-ATG8a in plant cells located in maturation zone of the primary root were observed 472 using a Leica TCS SP8 confocal microscope, GFP was imaged with 488-nm excitation 473 and a detection window of 500 to 530 nm. .. 474 Yeast two-hybrid assay 475 The coding sequences of genes were cloned into the pGBKT7 and pGADT7 vectors, 476 respectively, to investigate protein-protein interactions using the Matchmaker Gold 477 Yeast Two-Hybrid System (Clontech, CA, USA). ..

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens
    Article Snippet: .. Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech). ..



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    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
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    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) <t>Yeast</t> <t>two‐hybrid</t> assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.
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    Image Search Results


    Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Protein interactions between UvBhlh6 and other bHLH transcription factors. (a) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and UvBhlh1, UvBhlh4 and UvBhlh7. Yeast cells (10 2 –10 6 cells/mL) co‐transformed with prey and bait vectors were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Positive control: PGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative control: PGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam). (b) Bimolecular fluorescence complementation assay detecting interactions between UvBhlh6 and UvBhlh1, UvBhlh4 or UvBhlh7. YFP signals and DAPI staining were visualised using an LSM710 confocal microscope (Zeiss). Scale bar, 10 μm.

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Transformation Assay, Positive Control, Negative Control, Bimolecular Fluorescence Complementation Assay, Staining, Microscopy

    Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Journal: Molecular Plant Pathology

    Article Title: The Basic Helix–Loop–Helix Transcription Factors UvBhlh1 and UvBhlh6 Coordinate Virulence in the Rice False Smut Fungus Ustilaginoidea virens

    doi: 10.1111/mpp.70267

    Figure Lengend Snippet: Subcellular localisation and protein interactions involving UvBhlh6. (a) Co‐localisation of DAPI and UvBhlh1‐eGFP, UvBhlh4‐eGFP, UvBhlh6‐eGFP and UvBhlh7‐eGFP in mycelia. Scale bar, 10 μm. (b) Yeast two‐hybrid assay assessing interactions between UvBhlh6 and its putative interacting partners identified by affinity capture‐mass spectrometry. Yeast cells were spotted on SD/−Leu/−Trp and SD/−Ade/−His/−Leu/−Trp media. Controls: Positive, pGADT7 (AD‐T) + pGBKT7‐53 (BD‐53); negative, pGADT7 (AD‐T) + pGBKT7‐Lam (BD‐Lam).

    Article Snippet: Protein–protein interactions were analysed using the Matchmaker Gold Yeast Two‐Hybrid System (Clontech).

    Techniques: Y2H Assay, Mass Spectrometry